Lund University

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Chemical analysis of lignin

Supercritical fluid chromatography methods for lignin applications

Selectivity and efficiency in ultrahigh performance supercritical fluid chromatography (UHPSFC) are explored for analysis of lignin monomers, dimers and oligomers. A variety of polar stationary phases are investigated along with mobile phase composition (carbon dioxide, methanol and additives) and pressure, temperature and flow rate. Two-dimensional UHPSFC is also investigated as a separation technique for lignin applications.

High-resolution mass spectrometry

High-resolution mass spectrometry (HRMS) such as QTOF/MS and Orbitrap/MS are used in combination with ultrahigh performance liquid chromatography (UHPLC) and ultrahigh performance supercritical fluid chromatography (UHPSFC) to elucidate lignin monomer, dimer and oligomer chemical structures in complex lignin samples.

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